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Registration Number

0215U001125, 0112U006963 , R & D reports

Title

Structural changes of macromolecules and cells on the interaction with nanoparticles and biologically active substance.

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Head

Gordiyenko Evgen, Gatash Sergiy,

Registration Date

21-01-2015

Organization

Kharkov National University named after V.N. Karazin

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Objects of the study: DNA, chlorophyllin, bovine serum albumin, human serum albumin, nanodiamonds, hydroxyapatite nanocrystals, compound 1408, metformin, erythrocytes, platelets, blood serum. The aims of this work were to establish mechanisms of structural transformations of biological macromolecules and cells in interaction with nanoparticles and biologically active substances; to study the adhesive properties of the erythrocytes depending on the physico-chemical characteristics of the medium. Methods: molecular dynamics simulation, UHF and EHF dielectrometry, EPR spectroscopy, differential scanning calorimetry, photoelectrocolorimetry, microscopy, statistical methods. In this research we studied the structural changes of biological molecules and cells under the influence of factors of different nature, which play an important role not only in the normal functioning of living organisms, but also determine the development of irreversible pathological changes of macromolecular assemblies of cells, the consequences of which are, for example, mutagenesis and carcinogenesis. As a result, the temporal evolution of the different structural deformations of DNA complexed with the protein, in particular kink-type bending strain, was determined. First set: switching of the conformation of the deoxyribose in the nucleotide composition that contains cytosine to a C2'-endo C3'-endo conformation, i.e. В?А transition; deoxyribose conformation correlation with the magnitude of the angle ? glycoside polynucleotide chain; changes of angles of Propeller and Twist. It was found that formation of chlorophyllin complex with fatty acids free bovine serum albumins is accompanied by a significant decrease of hydration while no notable hydration changes takes place upon formation of chlorophyllin complex with human serum albumins containing fatty acids. The role of hydration of nanodiamonds in the stability of aqueous suspensions was confirmed. The existence of two states of water molecules in the nanocrystalline hydroxyapatite: adsorbed on the surface of the particles and hydrated was determined. The information about changes in the hydration and structure of human serum components under the influence of low temperatures and gamma radiation was obtained. The erythrocyte resistance to acid hemolysis in rabbits with the experimental model of dexamethasone type 2 diabetes by application of glucose-lowering agents was studied. It was found that the use of metformin leads to the approximation of the main indicators erythrograms erythrocytes of rabbits in this group to those of the control group erythrograms. The influence of different anticoagulants and storage time on the blood acid and hemolysis induced platelet aggregation was studied. It was found that the acid resistance of erythrocytes examined daily blood sampling is independent on the anticoagulant unlike indexes of platelet aggregation of the same blood. In in vitro experiments a negative impact of nanodiamonds on red blood cells which related to the reducing of the resistance of erythrocytes to hemolysis, was revealed. The contributions of electrostatic, hydrophobic and receptor-mediated interactions in the process of adhesion of bacteria on the surface of red blood cells were estimated. The results of research were introduced at the Institute for problems of cryobiology and cryomedicine of the National Academy of Science of Ukraine and may be used in the development of cryopreservation methods.

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Адельянов О.
Анікєєва М.
Горобченко О.
Жигалова Н.
Ніколов О.
Шаталова Т.
Шестопалова Г.

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2020-04-02