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Registration Number

0215U002481, 0115U005224 , R & D reports

Title

Selection of varieties of winter wheat Ukrainian selection for cold-acclimation, development of methods for assessing the level of gene expression of a-tubulin during cold acclimation.

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Head

Demkovych Andrii Yevgenovych, Кандидат біологічних наук

Registration Date

22-12-2015

Organization

Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine

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Primers for 15members of Triticum aestivum L. alpha-tubulin gene family were designed using PrimerBlast software. Cultivars with different levels of cold tolerans were selected: two winter cultivars (Mironovsky 808, Demetra) and two spring cultivars (Kharkovsky 26, Elegiya). Using choosen cultivars cold acclimation was conducted. Gene expression levels were measured using qPCR method. Ta_TUBA 2-3 gene was chosen as the first to measure the level of expression because of its potential role in cold tolerance. It was found that the level of Ta_TUBA 2-3 gene expression increases in the early stages of cold acclimation inwinter cultivar Demetra and spring cultivar Elegiya and decreases in winter cultivar Mironovsky 808 and spring cultivar Kharkovsky 26. Ta_TUBA 2-3 relative expression level in the later stages of cold acclimation was elevated in all four cultivars. Main differences between spring and winter cultivars were detected in alteration rate of Ta_TUBA 2-3 expression level: winter cultivars show a significantly greater speed of alterations.

Product Description

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Буй Денис Дмитрович
Демкович Андрій Євгенович

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2020-04-02