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Registration Number

0220U101300, 0119U001418 , R & D reports

Title

Nanozymes and nanomediators: screening, synthesis, characterization, the use in Biosensorics and enzymatic analysis based on microbial oxidoreductases

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Registration Date

07-02-2020

Organization

Institute of Cell Biology

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The synthesis of new conductive nanoparticles of noble and rare metals, including their hybrid forms, was carried out. Commercial and synthetic nanocomposite materials were screened for their peroxidase-like and electrochemical mediating activity in the solution and on the surface of the graphite electrode. Structural and functional characterization of the obtained nanomaterials using atomic force spectroscopy, X-ray spectral analysis, and scanning electron microscopy was performed. Peroxidase activity in the solution of nanocomposite materials based on cross-linked dextran and iron oxide (III) (up to 6.1 U⋅mg−1 of preparation) was shown, which simultaneously exhibit high chemosensory sensitivity to hydrogen peroxide at graphene electrode (41.1 A⋅M−1⋅m−2). Synthesis, functionalization and physicochemical, structural-morphological and catalytic characterization of materials based on porous carbon fibers (CF) modified by hemin, micro- and nanoparticles of precious metals were carried out. CFs modified with hemin and Au0 nanoparticles or Pt0 microparticles have been shown to exhibit 2-3 times better affinity and higher catalytic activity for H2O2 compared to free hemin. Mono- and bi-metal nanoparticles were synthesized on the graphite electrode surface by electrodeposition of 1 mm solutions of Pt4+, Pd2+, Ru3+, or Cu2+ salts in 0.1 M phosphate buffer, pH 7.0. The nanozymes (peroxidase-like) activity was analyzed using cyclic voltammetry. Hybrid bimetallic nPtRu nanoparticles were selected as nanocomposites with the highest peroxidase-like activity. Based on these, amperometric biosensors have been designed in combination with alcohol oxidase and methylamine oxidase to analyze primary alcohols and methylamine. Both biosensors have high sensitivity (336 A⋅M−1⋅m−2 for ethanol and 284 A⋅M−1⋅m−2 for methylamine, wide linear ranges (25-200 μM for ethanol and 20-600 μM for MA) and satisfactory storage stability.

Product Description

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Ivash Mariya F.
Gayda Galyna Z.
Honchar Mykhailo V.
Demkiv Olha M.
Zakalska Oksana M.
Zakalskiy Andriy Y.
Kutsyaba Vasyl I.
Prokopiv Tetyana M.
Serkiz Roman Y.
Smutok Oleh V.
Stasiuk Nataliya Y.

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2020-04-02