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Registration Number

0223U001547, 0121U108471 , R & D reports

Title

Research of peculiarities of ecological niche of anthrax pathogen and substantiation of measures for decontamination of anthrax burial grounds in Ukraine

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Head

Tarasov Oleksandr A.,

Registration Date

31-01-2023

Organization

Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine

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As a result of the research, it was established that the use of the germinant solution of the following composition: inosine - 0.35 mmol; L- histidine -0.5 mmol; L-valine - 0.3 mmol; L-serine -0.3 mmol and distilled water - up to 1 L specifically affects the germination of spores of bacteria of the genus Bacillus in vitro, while most of the inoculated spores germinated, forming vegetative forms of bacteria within the first two hours from the beginning of incubation. Analysis of the kinetics of germination under the studied conditions temperatures showed that the decrease in the optical density of the suspension, which indicated the germination of spores in the nutrient medium, after treatment with the germinant mixture reached values of 70.2±3.6% (B.cereus) and 63±7.1% (B.anthracis) according to temperature 8°C; 44.1±7.2% (B.cereus) and 39.5±5.6%% (B.anthracis) at a temperature of 15°С; 30.0±3.5% (B.cereus) and 38.7±7.2% (B.anthracis) at a temperature of 25°С; 21.3±6.0% (B.cereus) and 32.8±4.9% (B.anthracis) at a temperature of 35°С. At the same time, the highest rates of germination of spores germinated without the addition of a germinant ranged from 55.2 ± 8.2%% to 64.0 ± 5.6% at a temperature of 35°С. At lower temperatures, these values were at least 63-65%, which is almost half as much as in variants with the use of germinant and is statistically reliable. It was established that the optimal temperature for the growth of vegetative forms of bacteria with the use of germinant is in the range of 25-35°C, after 48 hours the growth reached maximum values - 8.4±0.4 log10 CFU/cm3 (B.anthracis) and 7.7 ±0.4 log10 CFU/cm3 (B.cereus).

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2023-01-31