Search academic texts

Registration Number

0209U010435, 0109U006159 , R & D reports

Title

Analysis of existent approaches to the use of surface plasmon resonance (PPR) for detection of intact viruses and their antigens. Development of the experimental conditions of funcionalization surface of sensor (procedures of funktsionalizatsii), in particular, study of influencie of additional surface charge on immobilization of protein molecules.

popup.stage_title

Head

Boltovets Praskoviya Mykolayivna,

Registration Date

03-12-2009

Organization

Institute of Semiconductor Physics of NAS of Ukraine

Description

A research object is sensor surfaces with the different type of functionalization. The purpose of work is development of approaches to creation on the surface of sensor of optimal conditions for deection of biospecific interactiobs at participation of viruses and their antigens . A research method is surface plasmon resonance (PPR). As current methods of modification of gold film (in particular, creation of carboximetildexran layer on its surface) do not allow in a complete measure to realize possibility of method, creation of intermediate layer between the explored protein and metal which would be simultaneously protective characteristics and ability to orient molecules near the interface is of both theoretical and practical interest. In particular, perspective is creation of the thin layer of small molecules on the sensor surface, which carry a certain charge which allowing to dispose the explored molecules near-by a surface are oriented, in dependence on their superficial charge. In accordance with it, within the framework of the stage approach which allows formation on a surface film of gold layer of molecules thiocianate K (КNCS) that form a thin buffer layer on a surface was developed. The results are basis for conducting of detection of the viral an antigen on the model system of tobacco mosaic virus - green algae with the use of specific anti-virus antibodies and protein A Staphylococcus aureus . The volume of work on the stage is executed fully.

Product Description

popup.authors

Кравченко Сергій Олександрович
Снопок Борис Анатолійович