Search academic texts

Registration Number

0216U001044, 0115U004185 , R & D reports

Title

Development, testing and manufacturing of trial series of enzymatic kit "Argitest" for arginine analyses in clinical samples. Part 1. Optimization of kit's composition, assay's protocol and development of scietific-technical documents.

Head

Gonchar Mykhailo, Кандидат педагогічних наук

Registration Date

14-01-2016

Organization

Institute of Cell Biology NAS of Ukraine

Description

The catalytic properties of recombinant arginine-selective enzymes, obtained in our institute from the cells of recombinant microbial producers ( bacterial ADI and three preparations of human liver arginase I), were investigated. Each of the studied enzymes was shown can be used as a component of enzymatic kit for L-arginine analysis. The kit "Argitest" with a fluorometric detection of the final product (urea) was developed and optimized. The kit is based on the simple, highly selective, sensitive and rather fast enzymatic-chemical method with the usage of urease, arginase and 2,3-butanedion monooxime. The kit "Argitest" was tested on the real samples of human serum; the ranges of normal and average values of L-arginine contents for healthy people were estimated. The obtained data was shown to correlate with the data of literature. The trial lot of kits was released and transferred in laboratory of biomolecular electronics of IMBG NAS of Ukraine for testing on real human blood samples of patients with different malignances. As result of research, 3 papers were published, 2 papers and 1 patent were fielded in print, 2 papers and 1 patent are in preparing now.

Product Description

popup.authors

Вовк Олена Іванівна
Гайда Галина Зуфарівна
Закальська Оксана Мирославівна
Закальський Андрій Євстахович
Кіт Юрій Ярославович
Маєвська Оксана Михайлівна
Мироновський Северин Леонідович
Сибірний Андрій Андрійович
Синенька Марія Миколаївна
Стасик Олег Володимирович
Стасюк Наталія Євгенівна
Стойка Ростислав Степанович
Фаюра Любов Романівна