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Registration Number

0224U031385, 0121U109291 , R & D reports

Title

The role of the adamantane derivatives in Pseudomonas aeruginosa biofilm formation and quorum-sensing regulation

Head

Vrynchanu Nina O., д.мед.н.

Registration Date

18-04-2024

Organization

Institute of Pharmacology and Toxicology of the National Academy of Medical Sciences of Ukraine

Description

 It was established for the first time that adamantane derivatives (AM-166, KVM-97), as well as azithromycin and ciprofloxacin, affect the Las, Rhl, and PQS QS systems of P. aeruginosa: the expression of genes regulating the synthesis of virulence factors, the activity of efflux pump and film formation. The effect of the compounds on biofilms is realized in the early stages of their formation, while that of azithromycin increases with increasing duration of exposure. The composition with azithromycin and methoxan shows a pronounced and long-lasting antibacterial effect. It was established for the first time that the disruption of the formation of S.aureus biofilms is realized by suppressing the expression of the genes ica, icaA and icaD, sarA, cidA, clfB, fib, finbB, ebpS, eno and by stimulating agrA, E. coli by suppressing the expression of fliC, motB, papC (AM -166) and increased expression of the fimA gene. Violation of energy processes in E. coli is associated with suppression of the expression of appB, appX, malP and malZ. AM-166 causes genomic variability (3 DNA fragments in the range of 400-500 bp and variability in the range of 1000-2000 bp).

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Ishchenko Liudmyla M.
Vyhovska Liliia M.
Humeniuk Nataliia I.
Zelena Liubov B.