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0420U101044,
Кандидат ветеринарних наук
20-07-2020
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Molecular diagnostics of porcine circovirus infection and the study of the phylogenetic relationships of its pathogen
Rudova Nataliia ,
popup.head Gerilovych Anton P.
popup.opponent Nedosiekov Vitalii V.
popup.opponent Sytiuk Mykola P.
popup.review Palij Anatolij P.
popup.review Sytiuk Mykola P.
popup.review Lymanska Olga Yu.
Дисертація присвячена вивченню молекулярно-генетичної структури та філогенетичних зв’язків популяції цирковірусу свиней ІІ типу, який циркулює на території України, та розробці методу його детекції на основі ізотермальної ампліфікації, придатного для застосування у польових умовах. Проаналізовано та узагальнено дані щодо поширення ЦВІС у світі та в Україні впродовж 2009–2019 рр. Проведено епізоотологічний скринінг та отримано нові дані щодо поширення ЦВС-ІІ серед поголів’я диких свиней в Україні. Установлено, що в Україні ЦВС-ІІ циркулює серед поголів’я диких свиней на рівні 31,8 %. Отримано нові дані щодо етіологічної структури цирковірус-асоційованих хвороб домашніх свиней у господарствах Східного регіону України. Уперше в Україні розроблено метод ізотермальної ампліфікації (LAMP) для детекції генетичного матеріалу ЦВС-ІІ та проведено адаптацію розробленого методу для застосування у польових умовах. Досліджено можливість використання ПЛР для генотипування ЦВС-ІІ. Уперше у світі проведено секвенування ділянки генома ЦВС-ІІ, що містить повну послідовність гена cap, із застосуванням технології нанопор. Вивчено молекулярно-генетичні характеристики популяції українських ізолятів ЦВС-ІІ та встановлено їхні філогенетичні зв’язки.
2020-07-20
2020-08-10
Updated: 2025-12-28
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