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Registration Number

0215U003387, 0110U005970 , R & D reports

Title

Research of order and biophysics-chemistry mechanisms of calixerens action on functioning of catalytic and transport proteins of biological membranes.

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Head

Kosterin Sergiy Oleksijovych,

Registration Date

28-01-2015

Organization

A.V.Palladin Institute of Biochemistry of National Academy of Sciences

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Laboratory studies of influence of row of calix[4]arenes on activity of Са2+- and Na+/K+ - transporting systems of biological membranes have been carried. Selectivity and affinity of their action have been studied. Project should be conducted during next few years. With the use of propidium iodide and flow cytometry method it was shown, that calix[4]arenes С- 97, С- 99 and С- 107 in the concentration of 100 nМ did not cause death of uterus myocytes both in the absence and presence of Ca2+ in the incubation medium. Thus investigated calix[4]arenes did not educe cytotoxicity. It is proven that previous incubation of myometrium cells with calix[4]arene C - 90 (100 µМ) in presence of 1 мМ of Са2+ is accompanied by the substantial decline of oxytocin-induced increase of Са2+ concentration in cytoplasm and reduction of speed of subsequent Ca2+ concentration decline. It was shown with the use of fluorescent probe of АNS, that calix[4]arenes С- 90 and С- 107 (selective inhibitors of calcium and natrium pumps of plasma membrane accordingly) able to be built in biological membranes and change their liquid-crystalline state and superficial charge. The level of mitochondria membrane polarization and Ca ion exchange in mitochondria are the key parameters reflecting functional state of these subcellular structures under normal and pathological conditions.Using flow cytometry and fluorescent dye TMRM it was shown that calix[4]arenes C136 and C137 (10 ?M) hyperpolarize myometrium mitochondria membranes. At maximum, the effect was 173% relative to the control. At the same time, calix[4]arene C138 did not influence the mitochondria membrane potential. The observed effect of calix[4]arene C136 and C137 on the level of mitochondria membrane polarization may be of practical importance in a situation when mitochondria membrane potential correction is required and in biochemical experiments.

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Бабіч Лідія Григорівна
Бевза Олександр Васильович
Векліч Тетяна Олександрівна
Данилович Ганна Вікторівна
Данилович Юрій Володимирович
Карахім Сергій Олександрович
Шкрабак Олександр Анатолійович
Шликов Сергій Георгійович

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2020-04-02