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Registration Number

0215U007052, 0114U004047 , R & D reports

Title

Molecular bases of creation biologically active and ecologically safe preparations with bioprotective and immunomodulative properties.

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Head

Galkin Anatoliy Pavlovich,

Registration Date

27-01-2015

Organization

Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine

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In the field and greenhouse conditions we have conducted investigations on the Peking cabbage plants of bioprotective action against parasitic nematode Heterodera schachtii Schmidt of new bioregulators: Averkom, Averkom nova-2, Violar and Phytovit, created on the basis of metabolite products of soil streptomycetes. It is shown that under impact of these bioregulators the increase of plant crop in 1,5-2,2 times and decrease of defeat of plants by nematode up to 25-70 % are observed. Using Dot-blot hybridization method the increase of difference in the degree of homology up to 27 - 49 % between the cytoplasmic mRNA and small regulatory si/miRNA from control Peking cabbage plants and from experimental Peking cabbage plants infected by nematode and treated with bioregulators was found. In the wheat embryo cell-free system of protein synthesis the increase of silencing activity up to 32-54 % of si/miRNA preparations, isolated from infected by nematode and treated with bioregulators plants, on the templates of nematode mRNA and plant mRNA, was shown.

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Іутинська Г.О.
Білявська Л.О.
Галкін А.П.
Галкіна Л.О.
Козирицька В.Є.
Циганкова В.А.

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2020-04-02