1 documents found
Registration Number

0220U101655, 0115U005201 , R & D reports

Title

Obtaining cell lines of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators

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Head

Spivak Svetlana I., Кандидат біологічних наук

Registration Date

17-02-2020

Organization

Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine

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 The new lines of potato plants (Solanum tuberosum L.) cultivar Vernissage with improved morphogenetic potential and genetically-mediated resistance to the parasitic nematode H. schachtii were obtained under in vitro conditions on nutrient media with bioregulators of microbiological origin used in concentrations: 25, 50, 75 and 100 μl/l. The viability index (%) of experimental potato plants-regenerants grown on the invasive background on nutrient media with bioregulators was found to be increased to 54-79 %, compared with control plants, among which the number of viable plants was 39 %. The degree of damage of the total leaf and stem surface in plants obtained on nutrient media with bioregulators is shown to be reduced to 31–48 %, compared to increase to 67 % degree of damage of control plants-regenerants. The dot-blot hybridization method showed the decrease to 36 – 65 % in the index of the hybrid cytoplasmic mRNA and si/miRNA molecules, isolated from cells of control and experimental potato plants-regenerants, grown under in vitro conditions on the artificial invasive background on nutrient media with bioregulators, compared with the same index of control plants. The experiments conducted under in vitro cell-free system of protein synthesis from wheat seedlings showed an increase in the silencing activity to 31 – 59 % on the template of plant mRNA of si/miRNA isolated from cells of experimental potato plants-regenerants, grown under in vitro conditions on the artificial invasive background on nutrient media with bioregulators, compared with activity of cytoplasmic si/miRNAs isolated from control potato plants-regenerants.

Product Description

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Iutynska Galyna O.
Biliavska Liudmyla O.
Spivak Svitlana I.
Tsygankova Viktoriia А.
Shysha Elena M.

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2020-04-02

R & D report
Obtaining cell lines of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators
Head: Spivak Svetlana I.. Obtaining cell lines of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators. (popup.stage: ). Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine. № 0220U101655
1 documents found

Updated: 2025-07-11


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