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Information × Registration Number 0119U103634, ( 0225U001090  0225U001182  0225U001366  0225U001635  ) R & D request Title Molecular mechanisms for quality control of translation with non-proteinogenic amino acids participation Head Tukalo Mykhailo A, Доктор біологічних наук Registration Date 06-11-2019 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 The goal of this project is to study the structural and conformational determinants of tRNAs, human leucyl-tRNA synthetase and alanyl-tRNA synthetases from various species that exclude the participation of non-proteinogenic amino acids such as norleucine, norvaline, homocysteine and D-alanine, in protein biosynthesis. The mechanisms of stereospecificity control in translation of genetic information have not yet been elucidated. Therefore, the ability of AlaRS and ProRS from different species (E. coli, Enterococcus faecalis and Homo sapiens) to edit D-amino acids will be studied. Additionally, separate trans-editing factor AlaX will be examined. The comprehensive approach, combining genetic engineering, biochemical, biophysical and molecular dynamics methods and quantum chemical calculations, will be applied. The study of the influence of incorporation of norvaline, norleucine and homocysteine into proteins on the growth of normal and cancer cells will be performed. The results of our investigations will be used for the search of new antibiotics against human pathogens and new drugs against cancer. Additionally, they also provide the basis for the creation of new orthologous tRNA-ARSase pairs for synthetic biology. popup.nrat_date 2024-12-10 Close