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Information × Registration Number 0120U100298, ( 0221U100295  0222U001248  0223U000278  0223U005810  0224U033583  ) R & D request Title Investigation of the role of сell signaling second messengers in action of phytohormones and their structural analogs. Head Kravets Volodymyr S., Доктор біологічних наук Registration Date 03-02-2020 Organization V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry of the National Academy of Sciences of Ukraine popup.description1 The project is devoted to understanding of the nature of primary cellular reactions to a number of phytohormones, polyamines and the role of phosphatidylinositol 4-kinases (PI4K) and EDS1 (ENHANCED DISEASE SUSCEPTIBILITY 1) proteins in production of cell signaling second messengers during the regulation of plant metabolism. Solving these goals anticipates the investigation of molecular mechanisms that transduce a phytohormonal signals in plant cells inducing a range of consequtive changes in intensity and direction of metabolic pathways leading to plant adaptation. Special attention will be paid to examination of the role of phosphatidylinositol 4-kinases and EDS1 proteins in formation of cell signaling second messengers in response to a range of phytohormones with application of transgenic Arabidopsis thaliana plants with mutation in phosphatidylinositol 4-kinases (PI4K) genes: pi4kβ1β2, sid2pi4kβ1β2, pi4kb1b2sid2eds1. Investigations of the role of phospholipases with changed expression of their isoforms are also planned in regulation of key enzymes of proline metabolism in response to polyamines. Analysis of specific responses of serine-rich peptides SCOOP is envisaged in regulation of cellular metabolism of Arabidopsis thaliana plants. Changes in phospholipid profile in Arabidopsis thaliana will be investigated during plant response to elicitors and hemi-biotrophic fungal infections with the help of genetically-inserted fluorescent markers. Investigations of the role of diacylglycerol kinases with changed expression of their isoforms will be also conducted in regulation of cell metabolism in response to polyamines. popup.nrat_date 2024-12-10 Close