Search academic texts

Registration Number

0120U102238, ( 0225U004153  0225U004154  0225U004155  0225U004156  ) R & D request

Title

Structural and functional studies of translation elongation factors of higher eukaryotes

Head

Negrutskyii Borys S., Доктор біологічних наук

Registration Date

28-04-2020

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

popup.description1

The aim of this study is to analyze the spatial organization and function of mammalian translation elongation factor 1 (eEF1A and the multi-protein eEF1B complex). To achieve this, the following tasks are planned. First, to obtain experimental data that will allow us to build atomic models of the spatial organization of the subunits of the eEF1B translation complex and the architecture of the complex as a whole. Second, to conduct experimental studies and to build the model of spatial organization of isoform 1 of elongation factor 1A based on them. Third, to determine the potential functional significance of methylation of lysine residues in the molecule of elongation factor 1A (eEF1A), in particular, to test the hypothesis experimentally that the methylation of some lysine residues in eEF1A may affect the interaction of this protein with partners , such as the subunits of the eEF1B complex, which may reveal the molecular mechanism of the recently discovered effect of eEF1A methylation on oncogenic cell transformation. Fourthly, describe the molecular mechanisms of action of novel eEF1A inhibitors with potential antitumor effect.

popup.nrat_date

2024-12-10