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Registration Number

0121U107836, ( 0225U003735  0225U003737  0225U003741  0225U003744  ) R & D request

Title

The substantiation of the principles of biochemical and DNA markers selection for the genotypes resistance assessment of Vitis vinifera L. grapes to fungal diseases agents

Head

Muliukina Nina А., Доктор сільськогосподарських наук

Registration Date

03-02-2021

Organization

National Research Center "VE Tairov Institute of Viticulture and Enology"

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The aim of the project is to identify and select effective biochemical and DNA markers for resistance screening of grapevine selection material (Vitis vinifera L.) to fungal pathogens. The project identifies grape powdery mildew as the main target of disease control treatments by pesticides in plant protection schemes and grapevine esca, a fungal trunk disease of perennial wood, one of the most effective means of control of which is the sanitary certification of grapevine planting material. Different degrees of the causative agents' research lead to the need of DNA identification use for the esca agents because in contrast to European vine-growing countries for Ukraine this question remains open. The use of molecular markers of resistance to esca will focus on biochemical markers, primarily grape stilbenes. To assess the resistance of grape genotypes to powdery mildew, microsatellite DNA markers of resistance genes will be used and the most resistant grape genotypes will be evaluated (according to the field resistance assessment) selected by NSC “Tairov Research Institute of Viticulture and Wine-Making». The second step in identifying molecular markers of resistance to powdery mildew will be grape polyphenolic compounds screening, the concentration of which may be a sign that can be quickly assessed in hybrid populations. The final result of the project will be the database of molecular markers of resistance to grapevine fungal diseases and guidelines for the use of this database in breeding programs for resistance to fungal diseases of grapes creation.

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2024-12-09