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Information × Registration Number 0125U002859, R & D request Title In Vivo Analysis of Stress-Induced Protein CoAlation in Drosophila melanogaster Head Bdzhola Anna V., Доктор філософії Registration Date 02-07-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 The aim of this study is to identify and characterize protein CoAlation as a poorly explored post-translational redox modification under in vivo conditions using the Drosophila melanogaster model. At the initial stage, we plan to evaluate the effect of circadian light regime changes on the extent and profile of protein CoAlation, followed by a comparative analysis with protein glutathionylation—another redox-dependent post-translational modification involved in the antioxidant response. The main objective is to investigate changes in CoAlation intensity under physiological conditions and in response to oxidative stress induced by three agents with distinct mechanisms of action: hydrogen peroxide, rotenone, and paraquat (the latter two are well-known inducers of Parkinsonism in biological systems). Additionally, we aim to assess protein CoAlation levels in homogenates of male and female Drosophila melanogaster imago to explore potential sex-specific features of this mechanism. To examine possible ontogenetic differences in the CoAlation response to oxidative stress, we will conduct experiments using third-instar Drosophila larvae, a developmental stage characterized by high metabolic activity and sensitivity to environmental stressors. popup.nrat_date 2025-07-02 Close