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Information × Registration Number 0200U004600, 0197U016740 , R & D reports Title The study of macromolecule hydration and mechanisms of their interaction with ionizing radiation popup.stage_title Head Maleev V.Ya, Registration Date 25-09-2000 Organization Kharkov National University named after V.N.Karazin popup.description2 The objective of the study - determination of molecular level mechanisms of hydration effect on conformational dynamics of helical nucleic acids, as well as, on hysteresis phenomena; the study of interaction of nucleic acids with antibiotic carminomicine; the study of fibrinogen conformational changes under action of low temperatures and gamma-irradiation. The objects of study - DNA, polynucleotides, antibiotic carminomicine, fibrinogen. The methods of study - mathematical modeling, gravimetry, absorption and fluorescence spectroscopy, dielectric spectroscopy. The mathematical models have been built for processes of sorption and conformational transitions induced with the change of water content of the DNA samples in whole and restricted cycles of relative humidity changes. The methods and algorithms have been developed to numerically estimate the conditions of stationary state existence and to describe the time evolution of NA-water systems. The absorption and fluorescence parameters of the homopolynu cleotide - carminomicine complexes have been obtained. The two competitive types of binding have been determined. The method for separation of the binding types has been suggested. The three conformational transitions of the fibrinogen molecule have been observed in the temperature range from 2 to 50 C. The data on the deep cooling and gamma-radiation effects on spectral and conformational properties of fibrinogen molecule have been obtained. Product Description popup.authors popup.nrat_date 2020-04-03 Close