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Information × Registration Number 0201U004333, 0198U005020 , R & D reports Title Antioxidant enzyme systems in normal and transformed cells popup.stage_title Head Velykyj Mykola Mykolajovych, Registration Date 20-03-2001 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Comparative study of activities of main enzymes of antioxidant defense in NIH3T3 (embryonic mouse fibroblasts) and L929 (mouse fibrosarcoma) cells has been studied. The lower activities of superoxide dismutase, catalase and glutathione peroxidase as well as higher glutathione reductase activity in transformed cells as compared with normal cells were observed. It was found that NIH3T3 cells were less resistant to oxidative stress caused by increasing hydrogen peroxide concentrations. Obtained data are in agreement with changes in intracellular level of malondialdehide in combination with 4-hydroxyalkenals (4-HNE), which has been used as an indicator of lipid peroxidation. Protein phosphorylation on tyrosine residues in NIH3T3 and L929 cells after exposure to 4-HNE and H2O2 was examined Product Description popup.authors popup.nrat_date 2020-04-02 Close