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Information × Registration Number 0201U004508, 0198U009013 , R & D reports Title The mechanism investigation of cell metabolisn in reproductive organs, oocytes and embryos and elaborate the stimulation methods of reproduction processes in cows and sows. To improve the composition of the incubation medium for fertilization i vitro. popup.stage_title Head Rozgoni Ivan Ivanovych, Registration Date 11-06-2001 Organization Institute of Animal Biology Ukrainian Academy of Agrarian Science popup.description2 Ассоunt about SEW: 58p., 28 tabl., 18 sources. Object of investigation- cows, heifers, secret of uterine glands, ovaries, cumulus-oocyte complexes.Purpose of the work- to investigate the composition of the uterine secrets, work out the hormonal-and vitamine preparate for development ansd getting alive of embryos, to create the optimal conditions for cultovation of oocyte-cumulus complexes under their maturing to metafase-2, to study the influence of the liposomal preparationon the intensity of biosynthetical processes in reproductive organs under oestrus stimylation in heifers. Methods of investigations- morphological, electrophoretical and spectrophotometrical. Results of investigations- In experiments on cows the uterine seeret unvestigation an different stages of cycle was conducted, the new hormonal-and-vitamin preparationdevelopment and getting alive of embryos was worked out. It was established that the addition of zinc ions to ration of cows under stimulation by hormone-and- vitamine prepara tio n increases the ovulatory and metabolic processes intensity in reproductive organs. The functional heterogeneity of condition in corns of uterus under one functional yellow corpus was determined. In experiment on oocyte-cumulus complexes (OCC) was established that insulin, estradiol, GP-P, GP-G, GP-K in certain doses had positive influence on the morphological characteristic of OCC, their RNA and DNA content and conduct to increase of cumulus ells quantity in OCC. Cell cytosols of ovaries and granulosa of follicles in low doses also increase of OCC quality, RNA and DNA contentnt.- Cultivation of OCC with follicles granulosa cells even more increases their quantity and the content the RNA and DNA in them.Complex introductions of oestradiol, GP-P and insulin in different combinations increase of OCC quantity and the content of nucleic acids in them. Addition to oestradiol and GP-P of essential amino acids methionine and lysine, in cultural medium for maturation of OCC gives rise to even more improvement o f OCC morphological characteristics and the content of nucleic acids in them. In investigations on heifers the influence of new liposomal hormone-and-vitamine preparations "OVOESTROGEN" and "OVOCOR" on reproductive organs under oestrus stimulation has been studied. The rise of biosynthetic processes in nuclei of reproductive and parenchy-matic organs was received. In the ovaries the quantity of growing and ovulated follicles and of yellow corpora were rised. Cows, heifers, reproductive organs, oocytes, cumulus, cultural mediums, cytozols, DNA. Product Description popup.authors popup.nrat_date 2020-04-02 Close