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Information × Registration Number 0201U005008, 0196U001913 , R & D reports Title To research molecular mechanisms of digestive processes in gastrointestinal tract of productive animals. popup.stage_title Head Mel'nychuk Dmytro Oleksijovych, Registration Date 18-05-2001 Organization National agrarian university popup.description2 Object of the research - cattle (age 3-5 years) and new-born calves (age 3 days and 1 hour, before colostrum feeding). Intention of the work - research digestion processes molecular mechanismes in the digestive tract cattle and new-born calves: study of the enzymes activity gydrolases and ATPases and also proteins composition of the plasmaleme enterocytes this animals and reveal relation of the new-born calves membrane proteins to the immunoglobulines reception. Methods of the research - acute experience; chemical (citrate/EDTA) method for the receipt isolate enterocytes; homogenization and centrifugation; biochemical methods for the enzymes activity definition intestines gydrolases and ATPases; electrophoreses and immunobloting membranes proteins. Searched the specific enzymes activity of hydrolases and ATPases and founded new proteins in enterocytes plasma membrane by small intestine of new-born cattle. On the basic of ability of proteins 120, 100, 87, 75 and 24 kD to make a immunoglobulines recepti on the hypotesis of receptor-endocitoses mechanism formation of colostrum immunity have been proposed. Product Description popup.authors popup.nrat_date 2020-04-02 Close