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Information × Registration Number 0204U000429, 0103U008704 , R & D reports Title Development and estimation of the completely automated analyzer based on a biosenror for determination of organophosphorus pesticides in agricultural products popup.stage_title Head Soldatkin Alexey, Доктор біологічних наук Registration Date 04-02-2004 Organization Institute of molecular biology & genetics popup.description2 The biosensor for detection of content of carbamate and organophosphorous pesticides in agricultural products was developed. Two enzymes (acetylcholine esterase and butyrylcholine esterase) as well as two types of transducers (conductometric planar electrodes and pH-sensitive field-effects transistors) were used. Minimal concentration that can be detected by the biosensor comprised 5x10-11 M for diisopropylfluorophosphate, 5x10-9 M for paraoxon ethyl, 3x10-7 M for trichlorfon, 5x10-6 M for paraoxon methyl, 2x10-6 M for carbofuran, и 5x10-6 M for parathion methyl. Analytical characteristics of the developed biosensors were optimized for the work with the model solutions. For the re-activation of the enzyme after inhibition pyrydyn-2-aldoxime metiodide, which is able of destroying catalytically inactive complex of diisopropylfluorophosphate ester and recover the enzyme active site, was used. The developed biosensor can be used as the "early-warning system" for detection of organophosphorous pesticides in agricultural products. The developed analytical method is very quick and simple. Product Description popup.authors popup.nrat_date 2020-04-02 Close