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Information × Registration Number 0204U000892, 0101U000853 , R & D reports Title Role of tumor necrosis factor and transforming growth factor beta in pathogenetic mechanisms and clinical course of malignant lymphoproliferative disorders popup.stage_title Head Matlan Volodymyr Lvovich, Registration Date 02-03-2004 Organization Institute of Blood Pathology and Transfusion Medicine of Academy of Medical Sciences of Ukraine popup.description2 The comparative studies of tumor necrosis factor (TNF) and transforming growth factor beta (TGF-b) have been conducted in 185 patients with different lymphoproliferative disorders (LPD). The significant increase of TNF and more slightly of TGFb plasma levels were revealed in LPD patients. The comparison of interleukins 6 and 10 contents in plasma and in cells supernatants from peripheral blood, lymph nodes and affected spleens have been performed in patients with non-Hodgkin's lymphomas (NHL). The stimulation of adhesive as well as proliferative features of human umbilical vein endothelial cells (HUVECs) by plasma and cells supernatants from NHL patients was shown. The peculiarities of recombinant TNF and TGFb influence on indices of HUVECs proliferation and adhesion have been established. Aim of the study was to determine the role of cytokines in tumor-host relationships in lymphoid malignancies. Object of the study - 185 patients with different lymphoproliferative disorders (LPD). Methods: biological using H3-thymidine and ELISA. Results: tumor necrosis factor (TNF) and transforming growth factor beta (TFR-b) levels in plasma and cells supernatants were determined in different LPD as well as interleukins-6 and -10 were estimated in non-Hodgkin's lymphomas (NHL). The influence both of TNF and TGF-b, plasma and cells supernatants from NHL patients on proliferative and adhesive features of HUVECs have been established. For the first time there was performed the comparative study of cytokines level in plasma and their production in cells supernatants both from tumor tissues and peripheral blood in patients with different LPD. The investigation of cytokines profile of affected spleen in comparison with malignant lymph nodes had been beginning in NHL patients. The influence of plasma and supernatants of different cells from NHL patients on HUVECs have been compared. Efficacy of the study consists in receipt of additional possibilities in prognosis of LPD and improvement of their treatment on the basis of prevention of their metastases and neoangiogenesis. Field of application - research institutes, hematological and oncological clinics. Product Description popup.authors popup.nrat_date 2020-04-02 Close