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Information × Registration Number 0204U003956, 0103U002338 , R & D reports Title Construction of recombinant yeast strains for detoxycation of heavy metals in waste waters and using them as biosensors. popup.stage_title Head Sybirnyj Andrij Andrijovych, Registration Date 03-06-2004 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Isolation, functional analysis and sequencing of GSH1 gene, involved in the regulation of glutathione biosynthesis, in methylotrophic yeast H. polymorpha were carried out. It was shown that H. polymorpha GSH1 gene is homological to Saccharomyces. cerevisiae MET1 gene encoding S-adenosyl-L-methionine uroporphyrinogen III methyltransferase. Transformants with cloned GSH1 gene were obtained and their physiological and biochemical properties were studied. It was shown that Gsh1+ transformants restored growth and ability to synthesize glutathione on GSH-deficient media in the presence of different carbon sources, including methanol, and acquired resistance to cadmium and chromate ions. Unique coiled-coiled structure, which belong to leucine-zipper transcription factors, and motifs responsible for multiprotein complex formation were found in N-terminus sequence of HpGsh1p. It was assumed that Gsh1 protein acts as a transcriptional factor of GSH2 gene encoding the first and limiting enzyme of glutathione biosynthesis, gamma--glutamylcysteine synthetase. Product Description popup.authors popup.nrat_date 2020-04-02 Close