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Information × Registration Number 0206U000209, 0103U000682 , R & D reports Title Research of on ultraviolet irradiation and photosensibilization virusinactivation action on RNA- and DNA-containing viruses in blood plasma. popup.stage_title Head Timchenko A.S., Registration Date 18-01-2006 Organization Kiev Research Institute of Haematolojy and Blood Transfusion popup.description2 Subject of investigation: blood plasma, RNA- and DNA-containing viruses,photosensitizers, cell culture. Methods of investigation: spectophotometrical, virusological, citological, chromatographic, electrophoregraphic. Donor blood plasma viruses inactivation regiments were investigated, these regiments allowed keeping plasma protein structure and physiological characteristics. Investigations included study of methylene blue (MB) absorbtion spectrum in two spectrum regions, they showed extent of interaction between photosensitizer molecules, plasma protein molecules and model viruses (simple viruses - poliomyelitis viruses and complex viruses - vesicular stomatitis viruses). The most effective dosage diapason for donor blood plasma photosensibilisation was selected (from 0,00002 to 0,0002 mol/L). Optimal photosensitizer concentration doesn't activate MB dimerisation. Blood plasma absorbtion layer thickness for wave-length 254 nm was detected; influence of different MB dosages (genotoxic influence) and virus inactivation agents (MB and ultraviolet (UV) illumination) on electrophoretic activity of donor blood virus inactivated plasma proteins was investigated. The most effective method of removing photosentizer molecules from donor blood plasma was detected. On the basis of invastigations the UV-equipment model forblood plasma irradiation was made up. Medical effect. Medicine. Product Description popup.authors popup.nrat_date 2020-04-02 Close