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Information × Registration Number 0206U000247, 0103U008711 , R & D reports Title Heterochromatin structural organization, its variability and influence on the genome dedifferentiation and reorganization processes in plant tissue culture in vitro popup.stage_title Head Kunakh Viktor Anatoliyovich, Registration Date 18-01-2006 Organization Institute of molecular biology & genetics popup.description2 nuclei the rye genome areas, containing highly repetitive sequences were shown to be organized as loop domains of stable regular size resultant from the DNA attachment to nuclear matrix. Cell transition from the proliferatively active state to differentiation was found to be accompanied with the changes of the domain cleavage. The specificity of the amount and distribution of the constitutive heterochromatin within the interphase nuclei and metaphase chromosomes among the various lines of maize were revealed. Positive correlation between the heterochromatin amount and ability to callus formation and growth in vitro was found to occur. Examination of the structure and variability pattern of the R.serpentina genome labile reiterated sequence revealed parallelism between the rearrangements of the latter in vitro and its changes to occur during speciation. That suggests the non-random nature of the rearrangements in cultured tissues of the genome region involved which may result from the peculiarities of its structure. In the course of dedifferentiation upon callus formation and further culturing in vitro C.capillaris genome undergoes the changes in chromosome morphology, heterochromatin block localization as well as rearrangements in fraction of repeated DNA sequences. Cell differentiation in ontogenesis and C.capillaris tissue culturing in vitro were found to induce changes in nucleotide modification pattern, in particular cytosine residues methylation pattern. Product Description popup.authors popup.nrat_date 2020-04-02 Close