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Information × Registration Number 0206U000526, 0106U000446 , R & D reports Title Investigation the biological role of adapter protein Ruk in the control of proliferation, differentiation and apoptosis in human cells popup.stage_title Head Drobot Lyudmyla Borysivna, Доктор біологічних наук Registration Date 30-01-2006 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The cloning of Ruk adaptor protein was performed into expression vectors of insect and mammalian cells. It was shown that recombinant proteins expressed in indicated expression systems. The methods of protein purification were developed from lysates of insect cells and lysates of transfected НЕК293 cells. The monoclonal antibodies to N-terminal SH3A domain of Ruk adaptor protein was developed and characterized. The investigations of multiple endogenous Ruk variants expression, its ability to formation of oligomeric complexes and subcellular distribution were performed in cell lines of various tissue origins. It was shown that expression level and localization of Ruk adaptor protein are specific for each of investigated cell lines. The expression profiles of multiple molecular Ruk form were analyzed by monoclonal antibodies in brain tumor of glial origins. The transcripts with sizes 3,5 kb, 2,5kb and 1,5 kb corresponding to Rukl, Rukm та Ruks isoforms were revealed. These isoforms were identified as immunoreactive bands with molecular weights 85, 56 та 16 kDa respectively. Oscillations in the ability of Ruk praline-rich motifs to interact with exogenous ligands were revealed, suggesting the existence of the dynamic equilibrium between active (opened) and inactive (closed, resulted from intramolecular interactions) forms of adaptor protein. Product Description popup.authors popup.nrat_date 2020-04-02 Close