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Information × Registration Number 0206U000620, 0101U002309 , R & D reports Title To develop methods of bovine leucosis diagnosis using immunological and molecularbiological assay popup.stage_title Head Vitaliy A. Sinitsyn, Доктор ветеринарних наук Registration Date 31-01-2006 Organization The Institute of veterinary medicine popup.description2 Report on research work: 50 pages, 11 tables, 4 figures, 14 saurces, 2 appendices. Object of research - enzymoimmunoassay, bovine leukosis virus, blood sera. Goal of the work - to develop the technology of diagnosticums manufacture for enzymoimmunoassay in bovine leukosis. Research methods - immunologic, serologic, biochemical and chromatographic methods; equipment - chromatography columns, polystyrene plates, sorbents. Results of research - the technology of bovine leukosis virus purification and concentration has been developed on the basis of adsorption and molecular sieve chromatography on macropore sorbens; optimal conditions of enzymoimmunoassay carrying out for the detection of specific antibodies to leukosis virus have been worked out and the order of reaction results registration has been determined; the antigen's working dos, pH, time and temperature values during sensitization of microplates and incubation of serum with adsorbed antigen have been determined. In Ukraine for the first time thetechnology of manufacture and use of diagnostic kits for leukosis using ELISA has been developed. A patent of Ukraine for invention has been issued. Laboratory specimen of the kit was produced which was subjected to interdepartmental testing and demonstrated 100% sensitivity and specificity. The results of research work will be used in practical work of laboratories. The development corresponds to the international level. Product Description popup.authors popup.nrat_date 2020-04-02 Close