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Information × Registration Number 0206U000665, 0102U000614 , R & D reports Title Study of the mechanisms of transient metals' (Fe, Cu, Cr) bioasimilation in non-conventional yeast s and divelopment on their base of the new bioanalitical methods. popup.stage_title Head Gonchar Mykhailo Vasyliovych, Кандидат педагогічних наук Registration Date 02-02-2006 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The main results of the work: a collection of the mutants of non-conventional yeasts, resistant to chomate and selenite ions, impaired in vacuolar function, and with modified ability to utilize L-lactate was created;physiological, biochemical, and genetic properties of the isolated mutants were studied; using the mutants as the models, it was shown that one of the mechanisms of yeast cells' resistance to selenite is an increased accumulation of selenium in the cells (up to 3500 ppm), including, elementary form Se0; for the first time, it was shown that one of the mechanisms of chromate detoxification by the yeasts is its extra-cellular reduction to the less toxic chromium(III); approaches were developed for cloning of genes responsible for the resistance to the oxyanions of chromium and selenium, as well as for utilization of L-lactate; gene-engineered overproducers of formaldehyde dehydrogenase of Hansenula polymorpha were constructed. Product Description popup.authors popup.nrat_date 2020-04-02 Close