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Information × Registration Number 0206U004301, 0104U008981 , R & D reports Title Studying of the light-responsive circadian clock discovered in human blood lymphocytes: molecular nature of the photoreceptor popup.stage_title Head Gamaleia Nikolai Fedorovoch, Registration Date 16-02-2006 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology popup.description2 The project authors have recently shown presence of a self-sustaining circadian clock in lymphocytes from human blood placed in culture. Responsiveness of the clock to visible light of certain wavelengths, especially red, implied that lymphocytic cells contained some photoreceptive substance. In the project, methods of the substance isolation and purification were elaborated, and its protein nature was established. Physicochemical, biochemical and immunological studies of the protein showed its likeness to serum albumin and allowed to demonstrate its fairly firm connection with a cell membrane. To function as a photoreceptor, the protein should contain a certain chromophore component. The various spectroscopic investigations, including light-absorbance, fluorescence, atomic, circular dichroism and also electron spin resonance, have revealed the tetrapyrrolic structure of the chromophore component in the photoreceptive protein. Thus, in the studies under this project, the general structure of the chromoproteid which mediates photosensitivity of human blood lymphocytes was elucidated.' Product Description popup.authors popup.nrat_date 2020-04-02 Close