Search academic texts

Information × Registration Number 0206U004559, 0105U006037 , R & D reports Title Development of the tecnology of preparative production of pharmacological recombinant proteins in plants by the example of human somatotropin popup.stage_title Head Kuchuk Nikolay Viktorovich, Registration Date 01-03-2006 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 The protocol for effective transient expression of foreign genes in plants was developed and high-scale infiltration of N. benthamiana and N. excelsior with Agrobacterium strains harbouring vectors including human growth hormone gene was performed. Recombinant somatotropin was detected in crude extracts of plant tissue and its identity was proved by immunological and electrophoretical methods. The protocol of extraction and partial purification of recombinant somatotropin using Q-sepharose anion chromatography was developed. Product Description popup.authors popup.nrat_date 2020-04-02 Close