Search academic texts

Information × Registration Number 0206U005301, 0104U009042 , R & D reports Title To conduct cytogenetic analysis material, created in vitro for the needs of varietial and heterotic breeding, to de fine genetic determination of carrot and beet signns and to conduct identification of parental pairs for F1 hybrids development popup.stage_title Head Ivchenko Tania Vladimirovna, Registration Date 14-03-2006 Organization Institute of vegetable and melons Ukrainian Academy of Agrarian Sciences popup.description2 In the result of conducted investigations there are selected optimal conditions of DNA isolation and defined system of RAPD-primers for definition intervariental polymorphism of bulb onion, head cabbage, tomato, table beet, radish, garden radish and carrot. There is created the collection of native polymorphous DNA of vegetable plants species of the Institute breeding (340 samples). Conditons are selected for conduction RAPD-PCR reaction and electrophoretic divition of products of amplification in agar and polyacrylamide gels. Methodic trials are conducted on electrophoretic divition of globulins of cabbage, table beet, garden radish and radish. There is revealed intra- and intervariental polymorphism of reserve albumins and PCR products, which potentially allows to introduce a genetic passport systems, to evaluate variety purity and biotypic composition of varieties and hybrids of the studied vegetable plants species. To improve the method for definition the ploidy level of the initial vegetable material, created under in vitro conditions,there are selected: the time for getting samples, exposition and concentrations of substances for pre-treatment of the material, that in the totak allowed to get preparations of the necessary quality. There is conducted the cytologic control of bulb onion, hesd cabbage, tomato and carrot plants with the aim to determine correlation connections between the development of reproductive organs and the output of plants haploid forms in culture in vitro, buds dimensions are determined, which answer the optimal for andro- and gynogenesis phases of pollen development in the studied species of plants. KEY WORDS: CARROT, TABLE BEET, GARDEN RADISH, HEAD CABBAGE, TOTAL DNA, RESESVE ALBUMINE (11S AND 12S GLOBULINS) MOLECULAR-GENETIC MARKERS, POLYMORPHISM, GENETIC DIFFERENTIATION, CLASTER ANALYSIS, UPGMA-DENDRORRAM. Product Description popup.authors popup.nrat_date 2020-04-02 Close