Search academic texts

Information × Registration Number 0207U004483, 0102U002460 , R & D reports Title By means of genetic engineering obtain new strains of nitrogen fixing microorganisms and create highly efficient nitrogen fixing systems of legumes and cereals popup.stage_title Head Kots Sergiy Yaroslavovych, Registration Date 03-04-2007 Organization Institute of Plant Physiology and Genetics NASU popup.description2 The research presents continuation of fundamental investigations of Institute of plant physiology and genetics of NAS of Ukraine devoted to the study of the mechanism of biological fixation of molecular nitrogen and activities development on the enhancement of atmospheric nitrogen binding level by biological means. More than 600 genetically-modified strains of nodule bacteria Sinorhizobium meliloti, Rhizobium leguminosarum bv. trifolii, Rhizobium leguminosarum bv. viciae were obtained by means of transposon mutagenesis. Inoculation of plants with transposants named above has resulted in vegetative mass yield increase of alfalfa and clover on 9,5 - 17% and pea grain yield on 12 - 17% comparing to productive (standard) strains. Plasmid pSUP2021 bearing Tn5 can be used also for transposon mutagenesis in nodule bacteria of lupine Bradyrhizobium sp. (Lupinus) and soy-beans Bradyrhizobium japonicum. By means of analytical selection the isolates of nitrogen fixing microorganisms from the rhizosphere of winter and spring wheat were secured and used as the basis of new combined bacterial preparation Coctail for presowing cereal seeds bacterization.5635 Product Description popup.authors popup.nrat_date 2020-04-02 Close