Search academic texts

Information × Registration Number 0207U008757, 0105U000300 , R & D reports Title Detection of indirect genetic instability development in blood lymphocytes of cancer patients undergoing radiation therapy. popup.stage_title Head Maznyk N.A., Registration Date 20-12-2007 Organization Grigor'ev Institut for Medical Radiology popup.description2 Matter of research: cytogenetic end-points of non-targeted genome destabilization occurrence of cultured human blood lymphocytes after therapeutic irradiation. Objectives: To examine the pathways of radiobiological phenomena of delayed genomic instability and bystander effect at chromosomal and sub-cellular level on the basis of the analysis of the changes of cytogenetic parameters in lymphocytes of radiotherapy patients. Methods and equipment: cytogenetic analysis of cultured human blood lymphocytes, microscopes Biolam I. Results and their novelty: For the first time the decline of the indirectly-induced genomic instability level in vivo has been shown in lymphocytes of patients with uterine body cancer during 1-2 years after radiotherapy. Simultaneously, the preserving of enhanced chromosome fragility in cells proliferating in vitro was found. For the first time the variability of clastogenic activity of blood plasma has been estimated using the test-system for cytogenetic assessment of the bystander effect in a group of patients before and after radiotherapy. For the first time a different cytogenetic sensitivity of cells in the first and subsequent mitoses during bystander effect incidence and importance of considering the test-lymphocyte donor’s individual responsiveness has been shown. For the first time the role of the radiation-induced genetic background has been assessed in compare with the value of the initial intrinsic cytogenetic status in formation of non-targeted effects in cancer patients’ lymphocytes in late time after radiotherapy. The area of application: radiobiology, oncology, radiotherapy. Product Description popup.authors popup.nrat_date 2020-04-02 Close