Search academic texts

Information × Registration Number 0207U008838, 0107U002580 , R & D reports Title Marking of mouse cells at early embryogenesis stages with fluorescent protein gene on the way of creating transgenic animals popup.stage_title Head Butenko Gennadij Michajlovich, Registration Date 24-12-2007 Organization Institute of Gerontology Acad. Med. Sci Ukraine popup.description2 Object - C57BL/6, CBA mice and their embryos on the pre-implantation stages of. Purpose - to obtain mice embryos, to mark them by fluorescent protein gene on the early stages of embryogenesis and to estimate gene expression during embryogenesis both, in the embryo culture and in the living. Results - it has been shown that super-ovulation stimulation of female mice for receipt of embryos on the pre-implantation stages can be induced by a combined injection of "Folligon" and "Chorulon". Micro-manipulator and inverted microscope were modified for working with embryos on the early stages of development. Micro-injection of GFP gene into the pronucleus was performed using the micromanipulation nanomechanotronic complex. Genetically modified and ordinary embryos were transplanted to pseudopregnant. Possibility of cultivation of mice preimplantation embryos is outside of organism to the morula stage. Product Description popup.authors popup.nrat_date 2020-04-02 Close