Search academic texts

Information × Registration Number 0208U002971, 0103U004745 , R & D reports Title Development of own molecular-biological diagnostic systems for detection of latent viruses and transgens with the purpose of obtaining of genetically modified plants and its cloning on the viruses-free basis. popup.stage_title Head Maksym Melnychuk, Registration Date 06-03-2008 Organization Natural-humanitarian scientific-educational institute NAU of Ukraine. popup.description2 Object of research - obtaining of new useful plants by methods of cell and genetic engineering. Diagnostics and identification of plant viruses. Interaction of alfalfa virus coat protein with RNA4 in vivo. The purpose of work - development of conception of a new breeding scheme. Development of systems for plant viruses identification. Creating of fix sterility forms of sugar beet plants. Demonstration of interaction of alfalfa virus coat protein with RNA4 in vivo. Methods of research - in vitro culture of plant tissues and protoplasts, methods of DNA isolation, electrophoresis and polymerase chain reaction, immune-enzyme analysis, Western blotting, DNA sequence. Results of the carried out researches - for the first time it has been shown an interaction of alfalfa virus coat protein with RNA4 in vivo. Conditions for sugar beet protoplast isolation were developed. Systems for potato viruses identification were created. A new approach to heterozygous plants reproduction was developed. Sphere of application - national biosafety, agriculture, pharmaceutics, food industry. Product Description popup.authors popup.nrat_date 2020-04-02 Close