Search academic texts

Information × Registration Number 0208U004387, 0105U003469 , R & D reports Title TRP-mediated temperature sensitivity in normal and pathology prostate epitelium cells. popup.stage_title Head Shuba Yaroslav M., Доктор біологічних наук Registration Date 06-06-2008 Organization International center of molecular physiology popup.description2 The human orthologue of rodent TRPM8 also appears to be strongly expressed in the prostate and in the prostate cancer-derived epithelial cell line, LNCaP. In this study, we show that despite such expression, LNCaP cells respond to cold/menthol stimulus by membrane current (I(cold/menthol)) that shows inward rectification and high Ca(2+) selectivity, which are dramatically different properties from "classical" TRPM8-mediated I(cold/menthol). Yet, silencing of endogenous TRPM8 mRNA by either antisense or siRNA strategies suppresses both I(cold/menthol) and TRPM8 protein in LNCaP cells. We demonstrate that these puzzling results arise from TRPM8 localization not in the plasma, but in the endoplasmic reticulum (ER) membrane of LNCaP cells, where it supports cold/menthol/icilin-induced Ca(2+) release from the ER with concomitant activation of plasma membrane (PM) store-operated channels (SOC). Product Description popup.authors popup.nrat_date 2020-04-02 Close