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Information × Registration Number 0209U002196, 0106U000869 , R & D reports Title Pharmacological analysis of potassium conductivity and intracellular ionized Ca2+ concentration changes in vascular wall effector elements under oxidative stress popup.stage_title Head Soloviev A., Registration Date 22-01-2009 Organization Institute of Pharmacology and Toxicology Academy of Medical sciences of Ykraine popup.description2 The effects of whole body irradiation on large conductance Ca2+ - activated K+ channels (BKCa) function and mRNA expression in rat thoracic aorta smooth muscle cells (SMCs) were studied using combined patch-clamp technique in whole-cell modification and RT-PCR analysis. Outward currents through BKCa channels in SMCs obtained from irradiated animals on the 9th and 30th days post-irradiation demonstrated a significant decrease of K+ current density amplitudes. Significant inhibition of maximal endothelium-dependent relaxation and shift of the dose-response curve to the right has been found in irradiated rats. It was shown that smooth muscle calcium sensitivity is increased following irradiation. No change of arterial pressure was found in irradiated guinea pig. Endothelium-dependent vasodilation was shown to be diminished on the 9th day and restored partly on the 30th day post-irradiation. The results of RT-PCR analysis shoved that expression both a-subunit and b1-subunit of BKCa channels appears to be considerably diminished on 30th day post-irradiation. Lipoflavon (phosphatidylcholine liposomes with quercetin) being added to the external solution restored endothelium-dependent relaxation in rat and guinea pig thoracic aorta. Lipoflavon being administrated intraperitoneal prevented arterial pressure increase in rats. Results of patch-clamp analysis demonstrated that phosphatidylcholine liposomes with quercetin increased outward potassium current. Effect of Lipoflavon is a sum of two components: action of phosphatidylcholine liposomes and quercetin. Peroxinitrite provoked decrease of vascular tone and had no effect on vascular endothelium-dependent relaxation. Product Description popup.authors popup.nrat_date 2020-04-02 Close