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Information × Registration Number 0209U005451, 0106U012406 , R & D reports Title Steam cells are basis for cell's biotechnology in future. popup.stage_title Head Tukalo Mykhaylo, Registration Date 17-02-2009 Organization Institute of molecular biology & genetics popup.description2 A new variants of feeder layer was created by genetic modification of cells which form feeder. The human LIF gene, with a sequence which encodes protein for an alarm peptid was cloned. His biological activityis was confirmed. The stably transformed variants of BALB3T3-A1 line was obtain by pC1-L and pC1-IL. Expression of human LIF by transfection eukaryotic cells and secretion of teir in an environment was point. Technology of production of skins equivalents, which can be utillized as an effective artificial skins substitutes at therapy of burns, and other skin destruction, is developed. Technology includes: (1) obtainment of cellular line 4BL2, which arise from human peripheral blood mesenchymal cells; (2) synthesis of copolymer hydrogels of acryl character; end (3) immobilization of human stem cells on the gel membranes. Thus, artificial coverages which can be utillized in clinical practice as an effective artificial leathercloth at treatment of massive burns and other skin defects, are created. Product Description popup.authors popup.nrat_date 2020-04-02 Close