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Information × Registration Number 0209U005802, 0106U011202 , R & D reports Title Connection of electrophysiological properties of a presynaptic neuron with postsynaptic responses in a synaptically-connected pair of CNS neurons popup.stage_title Head Veselovskiy Nikolay S., Registration Date 13-05-2009 Organization International center of molecular physiology popup.description2 There were two groups of GABA-ergic neurons found in hippocampal culture which differ by the type of electrical activity and kinetic characteristics of APs: fast- and regular-spiking. It was demonstrated that granular cells are characterized as regular-spiking, they possess calcium-binding protein somatostatin and combination of Kv1.2 & Kv4.2-type potassium channels. It was also shown that granular cells are able to evoke two types of excitatory postsynaptic currents differing by rise- and decay times and mediated by AMPA- and kainate types of glutamate receptors. Difference in characteristics of low-frequency AP series between excitatory and inhibitory hippocampal neurons were discovered, along with difference in combinations of voltage-gated potassium channels. This argues the difference in mechanisms of electroexcitability responsible for regular spiking ability in the neurons. Presence of high levels of Kv3.1 potassium channels and calcium-binding protein somatostatin was found to be a distinctivefeature for fast-spiking group of hippocampal interneurons. It was established that absence of delay-rectifying Kv1 potassium channels and presence of a slight fast-inactivating A-current are peculiar all inhibitory hippocampal neurons. It was shown that non-inactivating current is the main component of integral potassium current in GABA-ergic interneurons, and it results from activity of channels with -subunits of Kv7.2, Kv7.3 and Kv7.5 subtypes. Presence of Kv7-subfamily channels was proven also for axons of hippocampal interneurons; they were found to influence electrophysiological activity of the cells and GABA-ergic synaptic transmission. Finally, it was demonstrated that activation of Kv7-channels significantly depresses AP generation and, in turn, leads to decrease of quantity of GABA releases from terminals and reduction of synaptic response. Product Description popup.authors popup.nrat_date 2020-04-02 Close