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Information × Registration Number 0209U010006, 0108U002475 , R & D reports Title To develop and inculcate recommendation from technology of receip free of viruses material of merry and material of strawberry. popup.stage_title Head Kycher F. Nicolay, Registration Date 26-06-2009 Organization Institute of Pomology Name L.P.Symirenka popup.description2 A report is on NDR: 22 s, 2 tablas., 8 pictures. A research object is clonal subgrafts and matochno-coptovi trees of merry. A purpose of work is development and introduction of recommendations from technology of selection of highly productive bezsimptomnikh pestle plants, diagnostic verification of the selected plants with application of modern methods of diagnostics of viral infection and creation of pestle fund of free of viruses sorts and shdschep merry. Research methods are a visual selection, diagnostic testing of the selected pestle bushes and trees by the method of імуно-ферментного analysis (IFA) and with the use of biottests on grassy and arboreal plants-indicators. 15 sorta merrys were plugged in research: Khoroshavka, Nectar, Pokazkova, Legend of mliivska, Vasilisa, Valery Chkalov, Oleg, Exhibition, Coral, Prisadibna, Gift of Mlieva, Aborigenko, Turquoise, Slov'yanochka, Star and pestle plants of clonal subgraft of VSL-2. It was succeeded by sight to find out the conducted inspections the symptoms of defeat the viruses of circular plyamistostey in 30% from the amount of the inspected trees. Diagnostic testing the method of imunofermentnogo analysis (IFA) with the use of diagnostic sets to the viruses is set the defeat of necrotizing circular spotted (VNKP) - 66,6% trees a virus, by the virus of chlorotic circular spotted (VKHKP) - 22,2%, by the virus of wring of sheets of merry (VSLCH) - 17,75, by the virus of sharki plum (VSHS) - 6,6%. None of investigational standards was staggered the virus of chlorotic spotted of sheets of apple-tree (VKHPLYA). 3 45 testable the method of IFA of matochno-zhivcevikh trees of VSL-2 of defeat the above-stated viruses it is not discovered. Results are got largely correlated with the results of biottests on grassy plants indicators and on the arboreal indicator of Shirofudzhen. The inoculation of probed sortozrazkiv is also conducted on the subgrafts of VSL-2, which from data of previous researches is sensible to the viruses of circular plyamistostey. Absence of defeat of VNKP is set as a result of the conducted researches, VKHKP, VSLCH, VSHS, VKHPLYA of separate standards of sorts of Aborigenko, Coral, Legend of mliivska, Oleg, Pokazkova and Slov'yanochka allows, in obedience to the requirements of international standards, developed the commission of European organization of defence of plants (EOZR) for certifications testable on exciters of illnesses of sadivnogo material of fruit cultures, preliminary to subsume them "testable on viruses" material. New approaches are developed for the acceleration of receipt "free of viruses" of sadivnogo material. It is suggested to integrate in one technological process an express methods of diagnosticating of defeat of "parent" pestle plants and speed-up reproduction of the selected clonals of sortozrazkiv. KEYWORDS: MERRY, SHDSCHEPI, PRISCHEPNIY MATERIAL, VIROSISS, TESTING DIAGNOSTNCHNE, IMUNOFERMENTNIY ANALYSIS, ARBOREAL INDICATORS, SELECTION of BEZVIRUSNIKH of CLONALS. A report is on NDR: 15 s, 1 tablas., 6 pictures. A research object is the districted and perspective sorts of strawberry of domestic and foreign selection. A purpose of work is development and introduction of technology of receipt free of viruses of sadivnogo material of strawberry. Research methods are a visual selection, diagnostic testing by the method of IFA, by the method of bioppo6 on plants-indicators. It is set that considerable part of sorts of strawberry is staggered virosiss chronic character of which stipulates the necessity of development of prophylactic measures. The results of imunofermentnogo analysis (IFA) rotined absence of defeat of standards of sorts of Rozana of kiivska viruses, Rainbow, Present, El'santa, Khoney, Polka, Zenith, Rusich and in the separate standards of sorts Desna, Ol'viya, Dedication. Bci the probed standards of sort of Zenga Zengana and 1 standard of sort of Ol'viya were staggered the virus of mosaic of rezukhi, on one standard of sorts Desna and Dedication gave a positive reaction on the virus of circular spotted of raspberry. It was not discovered defeat, kil'cevoi spotted of sunini and black circular spotted of tomatoes, the viruses of latent. As a result of testing of biottests a method on plants-indicators four virosiss are diagnosed: wrinkliness of sheets, weak pozhovtinnya of edges of sheets, krapchastist' sheets and chlorotic oblyamuvannya veins. As a result of the conducted researches plants which can be taken to kateropii "testable on viruses" material are selected. As a result of speed-up reproduction matochnik is stopped up on plopc 0,5 hectares by a "weekend" by sadivnim material 11 sorts. KEYWORDS: SUNICYA, VIROSISS, DIAGNOSTIC TESTING, IMUNOFERMENTNIY ANALYSIS, BIOTTESTS ON PLANTS-INDICATORS, SELECTION of BEZVIRUSNIKH of CLONALS. Product Description popup.authors popup.nrat_date 2020-04-02 Close