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Information × Registration Number 0210U000114, 0109U006159 , R & D reports Title Development of the biosensor methods of intact viruses and their antigens diagnostics popup.stage_title Head Boltovets Praskoviya Mykolayivna, Registration Date 21-01-2010 Organization Institute of Semiconductor Physics of NAS of Ukraine popup.description2 The purpose of work on this stage is creation of sensitive layer on basis protein A Staphylococcus aureus as orienting component for carrying out imunospecific reactions with subsequent detection of viral antigen on the model system of tobacco mosaic virus -green algae by the methods of immunoferment analysis and SPR. Research methods are surface plasmon resonance (SPR), immunoferment analysis (IFA). Comparison an immobilization of protein A Staphylococcus aureus on the unmodified gold surface, surface modified by dodekanthiol, by the hydrogen sulfide and thiocyanat in different concentrations was carried out. It is shown that for immunoglobulins immobilization on the gold surface only sequential treatment of the surface by KNCS and proein A creates condiions necessary for oriented immobilization. By the methods of surface plasmon resonance, immunoferment analysis, light microscopy in lysates to the green algae Bracteococcus minor, artificially infected by tobacco mosaic virus, a viral antigen was found.The volume of work on the stage is executed fully. Product Description popup.authors Кравченко Сергій Олександрович Снопок Борис Анатолійов popup.nrat_date 2020-04-02 Close