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Information × Registration Number 0210U003344, 0106U000350 , R & D reports Title Investigation of the genetic features of mycobacterium of the tuberculosis and atypical mycobacteria, isolated from agricultural animals in the different regions of Ukraine and to develop PCR test-systems for their detection. popup.stage_title Head Zavgorodniy Andrey Ivanovich, Registration Date 01-02-2010 Organization National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" popup.description2 Research of clinical material is conducted from sicks and suspected on disease tuberculosis of animals, from which it is isolated exciters of tuberculosis of different kinds. DNA from bacterial mass of cultures is exstracted for the research in polymerase chain reaction (PCR) with the purpose of identification and differentiation. The analysis of variability sequences of IS 6110 of M. bovis and 16 S rRNA of M. avium complex is conducted. Conservative structural areas which allowed the design systems of primers for the indication of M. bovis and M. avium complex are determinated. Optimization of PCR - protocol of the design systems is conducted. In house PCR test-system for the indication and differentiation of mycobacterium of M. bovis and M. avium complex is constructed for the laboratory using. The development and improvement of method of the mycobacterium inactivation for the subsequent of molecular-genetic researches are conducted. Product Description popup.authors Герілович Антон Павлович Завгородний Андрей Иванович Сапко Світлана Анатольївна Скрипник Артем Валерьович popup.nrat_date 2020-04-02 Close