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Information × Registration Number 0210U003433, 0107U008765 , R & D reports Title The development and application of genetic technologies for the creation of genetically modified cells and organisms popup.stage_title Head Butenko Gennadij M.,Kordium Vitalii A., Registration Date 08-02-2010 Organization Institute of genetic and regenerative medicine Acad.Med.Sci. of Ukraine popup.description2 Technology of early stage development murine emryo isolation, micromanipulation and transplantation has been developed for their further genetic modification. Human LIF gene was cloned, producing strain for this protein was developed as well as construction for human LIF synthesis in mammalian cells was created. This should allow its secretion outside the cells. MSC isolation technology from umbilical cord was worked out. The isolated cells were characterized by morphology and surface markers specific for MSC. It has been shown that outside an organism all the characteristics change, and at the 3rd passage spontaneous adipo- and chondro- differentiation start. Phenotyping of hemopoietic and mesenchymal cells subpopulations from bone marrow, umbilical cord and fetal liver was performed by the method of flow cytometry. Procedure of hemopoietic stem cells sorting was developed for their further culturing and transplantation into the animals with model pathological states. Dissemination of GFP-labeled transplanted hemopoietic stem cells from bone marrow and fetal liver was shown in intact organism after short terms after transplantation. Evaluation of proliferative potental of own and donor cells in bone marrow of mice-recipients was done. Transplantation of GFP-labeled fetal neural cells was performed in animals with the model of cerebral ischemia. Dissemination and differentiation of donor cells in recipient brain was shown in dinamics. Methods for skin burns model in mice were suggested with the aim for further local or systemic introduction of GFP-labeled MSC for studying of perspective use of stem cells in regenerative medicine. Design of genetic cassetes was made, and recombinant plasmids for the production of hymeric proteins on the basis of ScFv and some partner proteins were constructed. Product Description popup.authors Дерябіна Олена Григорівна Лабунець Ірина Федорівна Павлова Марина Валеріївна Римар Світлана Юхимівна Скибо Галина Григорівна popup.nrat_date 2020-04-02 Close