Search academic texts

Information × Registration Number 0210U003491, 0107U004947 , R & D reports Title "The development of new diagnostic approach for testing of liver cell's alcohol damage". popup.stage_title Head Chaschin N.A., Registration Date 08-02-2010 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 In the animal model, developed by us, demonstrated that the chronic effect of ethanol leads to the intensification of free-radical oxidation of lipid and protein components of liver cells. Stressful damage occur in both the system and at the organ level. Chronic ethanol consumption is doubling the content of Cyp2e1 and Hsp90 in the liver. At the same time the level of ubiquitination of total protein in the liver of animals decreased approximately 2-fold compared with control. Using computational techniques we modeled "Cyp2e1/Hsp90" complex, which is characterized by high protein-protein binding energy level and large contact area, which showed Cyp2e1 and Hsp90 to be protein partners. These results allow us to offer a theoretical basis for the development test-system for determination of degree alcoholic liver injury by identify changes in expression level of Cyp2e1 and Hsp90 in comparison with the level of total ubiquitination of liver protein. Product Description popup.authors Бобик В.І. Кітам В.О. Максимчук О.В. Росохацька І.В. Рущак В.В. Сидорик Л.Л. popup.nrat_date 2020-04-02 Close