Search academic texts

Information × Registration Number 0210U006528, 0108U003277 , R & D reports Title Structural and functional investigation of the two tissue specific isoforms of eukariotic translation elongation factor eEF1A popup.stage_title Head Negrutskii B.S., Registration Date 29-09-2010 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 eEF1A crucially contributes to the efficiency of translation by providing correct binding of aminoacyl-tRNA to mRNA-programmed 80S ribosome. There are two 98% similar isoforms of eEF1A (A1 and A2) which are tissue and development-specific. A2, rather than A1, reveals oncogenic properties when appears in non-specific tissue (ovary, pancreas etc.). Why just one of the two 98% similar eEF1A isoforms reveals oncogenic properties remains unknown. Our aim is to unveil the molecular properties of eEF1A2 which could provide a molecular background for its cancer-specific action. We have shown different ability of the A1 and A2 isoforms to interact with SH2 and/or SH3 domains of different signaling molecules as well as with calmodulin which suggests a possibility of non-similar involvement of A1 and A2 in cell regulation. Striking conformational dissimilarity of the isoforms was found by X-ray scattering, сircular dichroism and confirmed by scanning microcalorimetry. A2 isoform behaves like a globular protein in solution while A1 possesses an extended conformation. Product Description popup.authors Верем"єва М.В. Лукаш Т.О. Новосильна О.В. popup.nrat_date 2020-04-02 Close