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Information × Registration Number 0210U007292, 0108U008389 , R & D reports Title Seeds of maize and sunflower. Identification methods of hybrid level by DNA-typing popup.stage_title Head Sivolap Yu.M., Registration Date 01-12-2010 Organization South Plant Biotechnology Center UAAS popup.description2 Seeds of the first generation with a high level of hybridity provides a manifestation of heterosis effect and a significant yield of maize and sunflower. Effective method of determining the level of first-generation hybrid seed is a DNA-typing by the existing, but still non-standardized, techniques. Score hybridity of seed lots by a comparative analysis of the spectra of amplification of microsatellite loci will reduce the time to assess and improve the accuracy of the installation of hybridity. Analysis of polymorphism of microsatellite loci allows to obtain stable codominant markers, with each locus, hybrid should get one allele from the maternal line, the second - from the parental. Electrophoretic spectra of plant hybrids should be identical and match the alleles of the original forms. By a comparative analysis of the spectra of amplification of individual seeds and counting the number of plants with the same allelic composition level hybridity of the seeds is set. Product Description popup.authors Сиволап Юрій Михайлович Солоденко Анжела Євгенівна popup.nrat_date 2020-04-02 Close