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Information × Registration Number 0210U007841, 0107U012139 , R & D reports Title To devise the methods of the direct acethylcholinergic neurons differentiation in mammalian brain stem cells culture for the treatment of neurodegenerative motor desease popup.stage_title Head Tsymbalyuk V.I., Registration Date 23-12-2010 Organization SI "Institute of Neurosurgery named after acad. A.Romodanov, AMS of Ukraine" popup.description2 46 pages, 26 pic., 18 ref. The object of investigation was human fetal nerve tissue. The aim of investigation was the induction of nerve stem cells direct differentiation to the cholinergic neurons. The methods of cell culture, immunocitochemistry and PCR was used. During experiment nerve cells of human embryo 9-10 weeks of gestation was tested for capacity to differentiate into cholinergic neurons. Using different neuroinductors and their combination we determined, that combination of acethilcholine and retinoic acid was most effective of them. We received 21% of ChAT-positive cells in the culture population. These cells expressed specific genes of cholinogenesis - VAChT and AChE and demonstrated the high level of AChE activity. The obtained data open new perspective for the cell therapy of neurological disorders. So, suspension of nerve cells, enriched by cholinergic neurons and their precursors can be received in culture and using as a material of neurotransplantation. Product Description popup.authors Васильєва І.Г, Галанта О.С. Олексенко Н.П. Сніцар Н.Д. Цюбко О.І. Чопик Н.Г. popup.nrat_date 2020-04-02 Close