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Information × Registration Number 0211U000359, 0106U001884 , R & D reports Title To eleborate biotechnologies of obtaining new maize genotypes tolerant to abiotic stresses and to use them in breeding and genetical investigations. popup.stage_title Head Satarova Tetjana Mikolaivna, Registration Date 01-02-2011 Organization Institute of Grain Farming of Ukrainian Academy of Agrarian Sciences popup.description2 Scientific investigations on elaboration of theoretical foundations of callusogenesis and regeneration in in vitro culture for perspective breeding maize genotypes, improvement of biotechnologies of obtaining regenerated plants for calli of certain types, elaboration of main principles of selection in in vitro culture for tolerance to abiotic factors and application of obtained results in breeding programs were fulfilled. For main commercial germplasms (heterotic groups) inbreds with high ability to morphogenic callusogenesis of different types were determinated which can serve the basic in their subgermplasms and germplasms for experiments on optimization of regeneration process, somaclone obtaining, purposeful genetic transformation of specific heterotic groups. Profound investigations of effects of such abiotic factors as low positive temperatures, high concentrations of sodium chloride, herbicide dicumba, aminoacids on callus tissue cultures in regeneration period and in embryoculture were carried out. The main methodic approaches to the creation of genotypes in in vitro culture tolerant to stress abiotic factors were elaborated. According to theoretical and practical developments biotechnologies of obtaining callus tissue of types I and II and plant regeneration were created, selective system for identification of genotypes tolerant to low positive temperatures on the ground of embryoculture was carried out, the biotechnology of preservation of elite and initial breeding materials in cold conditions was suggested, the method of obtaining cell cultures by direct and indirect techniques was improved. Obtained regenerated plants were transferred to breeding divisions as initial material. Induced callus cultures were used for search works on genetical transformation of maize lines of Ukrainian selection. Product Description popup.authors Абраімова Ольга Євгеніївна Деркач Катерина ВІкторівна popup.nrat_date 2020-04-02 Close