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Information × Registration Number 0211U001165, 0110U007302 , R & D reports Title Development of new technology for decrease of riboflavin B2 (riboflavin) production costs popup.stage_title Head Sibirny Andriy Andrijovych, Registration Date 20-01-2011 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The object of research: construction of riboflavin overproducing strains for biotechnological production of riboflavin (vitamin B2). The aim of research: developing of the industrial biotechnological obtaining of riboflavin using constructed riboflavin overproducing strains; parameters optimization for maximum riboflavin production. Methods: genetic engineering, molecular, genetic, microbiological, biochemical. Stabile strains-overproducers of riboflavin (vitamin B2) of the yeasts Candida famata were constructed using metabolic engineering methods. The metabolic engineering approaches involved introduction of additional copies of transcription factor SEF1 and IMH3 (coding for IMP dehydrogenase) orthologs from Debaryomyces hansenii, and the homologous genes RIB1 and RIB7, encoding GTP cyclohydrolase II and riboflavin synthetase, the first and the last enzymes of riboflavin biosynthesis pathway, respectively into C. famata AF-4 genome. Overexpression of these genes resulted in a 4-fold increase in riboflavin production. Improvement of medium composition resulted in additional 5-fold increase in vitamin B2 biosynthesis. Product Description popup.authors Дмитрук К.В. Федорович Д. В. Яцишин В.Ю. popup.nrat_date 2020-04-02 Close