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Information × Registration Number 0211U001829, 0106U000346 , R & D reports Title Create a recombinant strain of Marek's disease, to study the biological properties and use it to develop a DNA vaccine popup.stage_title Head Anton P. Gerilovych, Registration Date 04-02-2011 Organization National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" popup.description2 Research object - Marek's disease. The aim - to explore the genetic maps of Marek's disease agent, pick target genes responsible for synthesis of important immune proteins and to develop on the basis of recombinant structures, including a DNA vaccine and its immunogenicity study. Methods of research - molecular-genetic, bioinformatycal, microbiological, histological. When considering the restriction maps of the genome known epizootic and vaccine strains were modeled VHM plasmid vectors - carriers of pathogen genes Hmm. A selected primers and amplification system for these genes, optimized parameters of the reactions and restriction legiration and transfection of various donor test systems for the elaboration of recombinant DNA VHM. The technology of preparation of liposomal DNA complexes. Based on the vector was established model of DNA vaccine in liposome form. Performance laboratory was tested in interlaboratory studies and immunogenicity of innocence in experiments on chickens. The high protective properties of vaccines and for considering the restriction maps of the genome known epizootic and vaccine strains were modeled VHM plasmid vectors - carriers of pathogen genes Hmm. A selected primers and amplification system for these genes, optimized parameters of the reactions and restriction legiration and transfection of various donor test systems for the elaboration of recombinant DNA VHM. The technology of preparation of liposomal DNA complexes. Based on the vector was established model of DNA vaccine in liposome form. Performance laboratory was tested in interlaboratory studies and immunogenicity of innocence in experiments on chickens. The high protective properties of vaccines and harmlessness of the proposed liposomal forms. As a result of complex researches the production technology of DNA vaccines and regulations controlling the product quality, harmonized with modern regulations on accounting and recombinant DNA preparative forms of WHO, FAO, OIE. Industries: Veterinary Biotechnology Product Description popup.authors А. Герілович В. Болотін М. Стегній О. Лиманська О. Солодянкін П. Шутченко Р. Кучерявенко popup.nrat_date 2020-04-02 Close